2016 assigned starts are listed below. Day-of-race registrants will start after pre-registered riders, in the order that they sign up.
| Order | Bib. | Name | Team | Category | Start Time |
|---|---|---|---|---|---|
| 1 | 1 | DEMATOS Rebeka | Burn | U15 | 10:00:00 |
| 2 | 2 | GOODWIN Angelo | Putney West Hill Cycles | U15 | 10:00:30 |
| 3 | 3 | YANNELLI Everett | U15 | 10:01:00 | |
| 4 | 7 | GREEN Bob | Burn | 70+ | 10:03:00 |
| 5 | 8 | LECLERC Jean-Claude | 70+ | 10:03:30 | |
| 6 | 9 | MCCARTHY Ted | Team Burn | 70+ | 10:04:00 |
| 7 | 10 | COTNOIR Laura | Qvvelo | 60-69 Women | 10:04:30 |
| 8 | 11 | DANFORTH Alexandra | 60-69 Women | 10:05:00 | |
| 9 | 12 | TARDIFF Barbara | Qt2 Systems | 60-69 Women | 10:05:30 |
| 10 | 13 | COTNOIR Marc | Quinebaug Valley Velo Club | 60-69 Men | 10:06:00 |
| 11 | 14 | ERNEST Dan | Velorentides | 60-69 Men | 10:06:30 |
| 12 | 15 | GORDON Irvin | 60-69 Men | 10:07:00 | |
| 13 | 16 | GREEN Jonathan | Burn | 60-69 Men | 10:07:30 |
| 14 | 17 | HAENN Michael | 60-69 Men | 10:08:00 | |
| 15 | 18 | KELLY Dennis | Woodstock Cycling | 60-69 Men | 10:08:30 |
| 16 | 19 | LEWIS Rick | Body & Soul | 60-69 Men | 10:09:00 |
| 17 | 20 | MCCUSKER Michael | Northampton Cycling Club | 60-69 Men | 10:09:30 |
| 18 | 21 | MORIARTY Peter | 60-69 Men | 10:10:00 | |
| 19 | 22 | OTTEN David | 60-69 Men | 10:10:30 | |
| 20 | 23 | SORENSON Rick | Peak Racing/ Valley Bike & Ski | 60-69 Men | 10:11:00 |
| 21 | 24 | MCCLINTOCK Elizabeth | Cannondale Lingo Jingo | 50-59 Women | 10:11:30 |
| 22 | 25 | STEIN Carla | 50-59 Women | 10:12:00 | |
| 23 | 26 | MOTTA Aimee | 50-59 Women | 10:12:30 | |
| 24 | 27 | CLARK Kevin | Marti Shea Coaching | 50-59 Men | 10:13:00 |
| 25 | 28 | GODDARD Jonathan | 50-59 Men | 10:13:30 | |
| 26 | 29 | GREEN Rick | Burn | 50-59 Men | 10:14:00 |
| 27 | 30 | HALFREY Matt | 50-59 Men | 10:14:30 | |
| 28 | 31 | HAMMOND Marc | Fit Werx | 50-59 Men | 10:15:00 |
| 29 | 32 | MARTI David | 50-59 Men | 10:15:30 | |
| 30 | 33 | OLIVER Bim | 50-59 Men | 10:16:00 | |
| 31 | 34 | REEVES William | 50-59 Men | 10:16:30 | |
| 32 | 35 | RYAN Terrence | 50-59 Men | 10:17:00 | |
| 33 | 36 | SHIFFER Tom | Cadence Wealth Management | 50-59 Men | 10:17:30 |
| 34 | 37 | VANDENDRIES Erik | 545 Velo | 50-59 Men | 10:18:00 |
| 35 | 38 | HASKELL Kathy | Mad River Riders | 40-49 Women | 10:18:30 |
| 36 | 39 | NAGY Madeline | Ncc | 40-49 Women | 10:19:00 |
| 37 | 40 | ROUSH Caroline | Team Burn | 40-49 Women | 10:19:30 |
| 38 | 41 | DEMATOS Ricardo | 40-49 Men | 10:20:00 | |
| 39 | 42 | MACKENZIE Bruce | 40-49 Men | 10:20:30 | |
| 40 | 43 | OLIVER Sammy | 40-49 Men | 10:21:00 | |
| 41 | 44 | OLIVER Max | 40-49 Men | 10:21:30 | |
| 42 | 45 | PEABODY Chris | Team Fit Werx | 40-49 Men | 10:22:00 |
| 43 | 46 | SPINNEY John | Qt2 Systems | 40-49 Men | 10:22:30 |
| 44 | 47 | STEINBERG Gary | Brauer / Mick Management | 40-49 Men | 10:23:00 |
| 45 | 48 | DOUGGTY Alanna | 30-39 Women | 10:23:30 | |
| 46 | 49 | BROUILLETTE Kurt | Cyclonauts Racers | 30-39 Men | 10:24:00 |
| 47 | 50 | CARY Tim | Northampton Cycling Club | 30-39 Men | 10:24:30 |
| 48 | 51 | DALTON Harold | 30-39 Men | 10:25:00 | |
| 49 | 52 | KURLAND Jason | 30-39 Men | 10:25:30 | |
| 50 | 53 | YANNELLI Jason | United States Military Enduran | 30-39 Men | 10:26:00 |
| 51 | 54 | FENNO Jesse | 20-29 Men | 10:26:30 | |
| 52 | 55 | OWENS Edward | 20-29 Men | 10:27:00 | |
| 53 | 56 | MEYER Jonathan | 15-19 | 10:27:30 |